一、His抗體A00186產(chǎn)品介紹：

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.

THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.

THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.

THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.

中文：

特異于6個His標(biāo)簽的單克隆抗體可以極大地提高幾種不同免疫分析的有效性，幫助研究人員識別、檢測和純化細菌、昆蟲細胞和哺乳動物細胞中的多His融合蛋白。然而，由于6XHis標(biāo)簽的免疫原性較差，因此需要與KLH或其他載體結(jié)合作為免疫原。經(jīng)過數(shù)百個選擇周期后，GenScript的研究人員成功地分離出一種針對他的標(biāo)簽的抗體。

這個™ His標(biāo)記抗體mAb與小鼠（IgG1亞型）具有很高的親和力。在GenScript上進行的測試顯示抗體也能識別4xHis和5xHis標(biāo)簽。這意味著即使6xHis標(biāo)簽僅部分暴露，它仍將被該抗體識別和結(jié)合。

THTM His標(biāo)記單克隆抗體由小鼠腹水制備，并通過蛋白A親和柱純化。該抗體識別天然和變性形式的合成多His和多His標(biāo)記的融合蛋白。該產(chǎn)物與細菌、昆蟲細胞和哺乳動物細胞中表達的融合蛋白反應(yīng)。THTM-His標(biāo)簽單克隆抗體識別位于融合蛋白N端、C端和內(nèi)部區(qū)域的His標(biāo)簽。

THTM-His標(biāo)記單抗可用于培養(yǎng)細胞的Western印跡分析、斑點印跡分析、ELISA、免疫熒光染色和流式細胞術(shù)。

二、His抗體A00186產(chǎn)品特性：